The Most Trusted Name in Chromatography

Scientific Adsorbents provides the finest quality products and expertise available for chromatographic separation techniques. We specialize in the manufacturing and distribution of: Silicas, Aluminas, TLC Plates, and Desiccants from the analytical laboratory to the large scale process plant.

For over twenty years we have been committed to provide you with the highest quality products, the best customer service, at unbeatable prices!

What Is Chromatography?

Chromatography is a powerful analytical technique used to separate, identify, and quantify components in a mixture. Whether in pharmaceuticals, environmental testing, food safety, or biotechnology, chromatography enables researchers and industries to achieve unparalleled precision and accuracy.

Workflow of Chromatography


Chromatography is a methodical process used to separate and analyze the components of a mixture. Below is a step-by-step overview of a typical chromatography workflow:

Sample Preparation


  • Objective: Ensure the sample is suitable for the chosen chromatographic method.
  • Steps:
  1. Dissolve the sample in a compatible solvent.
  2. Filter or centrifuge to remove particulates.
  3. Adjust pH or ionic strength as needed.
  • Outcome: A homogenous sample ready for injection into the system.

Selection of Chromatographic Technique

  • Options:
  1. Gas Chromatography (GC): For volatile and thermally stable compounds.
  2. High-Performance Liquid Chromatography (HPLC): For non-volatile and thermally labile compounds.
  3. Thin Layer Chromatography (TLC): For quick, qualitative analysis.
  4. Affinity Chromatography: For biomolecular interactions like antibody-antigen binding.
  • Outcome: Optimal technique selected based on the sample and target analysis.

Stationary Phase and Column Selection


  • Considerations:
  1. Nature of the sample (polar or non-polar).
  2. Separation goals (e.g., size exclusion, ion exchange).
  3. Compatibility with the mobile phase.
  • Outcome: A column packed with the appropriate stationary phase is chosen

Mobile Phase Preparation

Objective: Facilitate the movement of sample components through the stationary phase.

  • Steps:
  1. Choose solvents based on polarity and compatibility.
  2. Degas the mobile phase to prevent bubble formation.
  3. Maintain consistent flow rate and pressure.
  • Outcome: A mobile phase optimized for separation efficiency.

Sample Injection


Method:Introduce the sample into the chromatographic system manually or via an auto-sampler.

Outcome: The sample enters the mobile phase and begins interacting with the stationary phase

Separation Process 


  •  Mechanism:
  1. Components of the mixture interact differently with the stationary and mobile phases.
  2. Separation is achieved based on properties such as size, charge, hydrophobicity, or affinity.
  • Outcome: Components are resolved and eluted at different times.

Detection and Data Collection


  • Detectors Used:
  1. UV-Vis Spectroscopy.
  2. Mass Spectrometry (MS).
  3. Fluorescence Detectors.
  4. Refractive Index Detectors.
  • Outcome: Signals are recorded as peaks on a chromatogram, representing separated components.

Data Analysis 


  •  Steps:
  1. Measure retention times and peak areas.
  2. Compare against standards for identification and quantification.
  • Outcome: Comprehensive analysis of the sample composition.

 Column and System Maintenance

  • Tasks:

  1. Flush the column with appropriate solvents to remove residual contaminants.

  1. Store columns under specified conditions.
  2. Perform routine checks and maintenance of the chromatographic system.
  • Outcome: Prolonged system and column lifespan, ensuring consistent performance.

Report Generation


  • Objective: Provide a detailed summary of the results.
  • Steps:
  1. Include chromatograms, retention times, and quantification data.
  2. Provide interpretations and conclusions.
  • Outcome: A comprehensive report ready for further use or publicatio



Products sorbents 

  • SAI's adsorbents for chromatography are manufactured to exact physical-chemical specifications to ensure reproducibility of the chromatographic process.
  • Continuous quality control ensures the availability of a standardized product that will provide reproducible results... day to day, lab to lab, process to process, worldwide. SAI's sorbents originate from the same starting material. Thus, the analyst can freely use small particles for HPLC,  HPTLC, and larger particles for Prep LC and Process. This allows one to utilize similar separation conditions to achieve the desired results and freely move from one technique to another.
  • Standardized alumina has become synonymous with Scientific Adsorbents, Inc. Never before has an adsorbent been more precisely standardized and allowed such a high degree of reproducibility when used in laboratory and plant operations.
  • This leadership lended its impulses to all other Scientific Adsorbents' products such as silica gels and products for Thin Layer Chromatography, Liquid Chromatography and the preparative link between TLC and Column Chromatography, Dry Column Chromatography (DCC).